Coding

Part:BBa_K3544409:Design

Designed by: 博宇 邹   Group: iGEM20_SCU-China   (2020-10-27)


OpbuCPV18,the 2A sequence with self-cleaving activity from Operophtera brumata cypovirus-18


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The effect of this 2A sequence on the three-dimensional structure of yeGFP at its C-terminal was simulated in dry lab


Source

Operophtera brumata cypovirus-18

References

Souza-Moreira, Tatiana M , et al. "Screening of 2a peptides for Polycistronic Gene Expression in Yeast." Fems Yeast Research 5:5.